Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Schematic representation of plasmonic fiberoptic absorbance biosensor (P-FAB) strategy for the detection of SARS-CoV-2 N-protein, photographic image oof GOF and POF sensor probes, and the LED-PD based experimental set-up used for the optical absorbance measurement.

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques:

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Dose-response curve obtained from POF sensor probes with capture antibody (Anti-SARS-CoV-2 N-protein IgG1) immobilized by means of physisorption, Acid-treatment, HMDA, and EDC/NHS based covalent binding methodology using a green LED-photodetector setup due to binding of immunocomplex with AuNP labels (40 nm, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$10\times $ \end{document} ) conjugated with anti-SARS CoV-2 -N-protein IgG2 (n = 3).

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Absorbance response obtained for a range of SARS-CoV-2 N-protein concentrations from POF and GOF sensor probes chemisorbed with anti-SARS-CoV-2 -N-protein IgG1 with the help of a green LED-photodetector setup due to binding of immunocomplex with AuNP labels (40 nm, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$10\times $ \end{document} ) conjugated with anti-SARS CoV-2 -N-protein IgG2 (n = 3). The width of the control band represents the standard deviation of respective POF and GOF sensor probes (n = 3).

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay, Standard Deviation

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: (A) Photographic images of AuNP conjugates of varying sizes subjected to NaCl stability test. Stable conjugates remain pink, while the AuNP labels with insufficient surface coverage agglomerates and turns violet or colorless. (B) Schematic representation showing the possible orientation of IgG on the AuNP surface. (C) Absorbance spectra of AuNP labels for varying sizes obtained using USB 4000 spectrometer. A broader full-width half maxima of the citrate-capped AuNPs indicates a 30-40% wider size distribution in comparison to 40 nm AuNP.

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques:

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: (A) Representative temporal absorbance response and (B) dose-response curves obtained for a range of SARS-CoV-2 N-protein concentrations from GOF sensor probes chemisorbed with anti-SARS-CoV-2-N-protein IgG1 with the help of the optical experimental setup due to binding of immunocomplex with AuNP labels varying sizes and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$10\times $ \end{document} concentration conjugated with anti-SARS CoV-2 -N-protein IgG2 (n = 3). Inset: TEM images of citrate reduced AuNPs prepared in-house.

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay, Concentration Assay

Journal: Ieee Sensors Journal

Article Title: Plasmonic Fiberoptic Absorbance Biosensor (P-FAB) for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

doi: 10.1109/JSEN.2021.3107736

Figure Lengend Snippet: Normalized absorbance response obtained from U-bent GOF sensor probes immobilized with anti-SARS CoV-2 IgG1 and * goat anti-HIgG due to specific and non-specific binding (NSB) of anti-SARS CoV-2 N-protein IgG2-AuNP labels with at varying concentrations (n = 3), respectively. A (C) refers to the average absorbance response obtained from the control experiments for NSB, while A (T) refers to the absorbance response obtained from each of the analyte concentrations (specific or non-specific).

Article Snippet: The bioreagents including mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG1 (capture antibody), and mouse anti-SARS-CoV-2 Nucleocapsid protein monoclonal antibody IgG2 (detector antibody) were purchased from Meridian Life Sciences, Inc., USA.

Techniques: Binding Assay